Table 3.
|
n |
EC50 (μm) |
pEC50 |
ϵ′ |
---|---|---|---|---|
WT | 6 | 17.38 | 4.76 ± 0.13 | 0.69 ± 0.03 |
H33A | 13 | 7.94 | 5.10 ± 0.08 | 0.66 ± 0.09 |
Q37A | 6 | 72.44 | 4.14 ± 0.05 | 0.26 ± 0.02** |
I40A | 5 | 1.51 | 5.82 ± 0.03 | 0.86 ± 0.03 |
L41A | 6 | 33.11 | 4.48 ± 0.02 | 0.32 ± 0.02** |
Y43A | 7 | 1.26 | 5.90 ± 0.13 | 0.90 ± 0.04* |
F44A | 11 | 1.00 | 5.99 ± 0.06 | 0.80 ± 0.03 |
I50A | 7 | 37.15 | 4.43 ± 0.09 | 0.33 ± 0.05** |
I328A | 5 | 1.38 | 5.86 ± 0.04 | 0.89 ± 0.01** |
P329A | 5 | 3.47 | 5.46 ± 0.11 | 0.66 ± 0.01 |
N333A | 7 | 4.07 | 5.39 ± 0.06 | 0.94 ± 0.02** |
L338A | 10 | 74.13 | 4.13 ± 0.11 | 0.28 ± 0.05** |
T339A | 6 | 8.51 | 5.07 ± 0.09 | 0.96 ± 0.03** |
S340A | 7 | 75.86 | 4.12 ± 0.07 | 0.31 ± 0.04** |
G342A | 5 | 63.10 | 4.20 ± 0.04 | 0.36 ± 0.01** |
V343A | 4 | 1.91 | 5.72 ± 0.13 | 1.01 ± 0.15** |
G344A | 8 | 91.20 | 4.04 ± 0.05 | 0.11 ± 0.02** |
S345A |
5 |
21.38 |
4.67 ± 0.13 |
0.42 ± 0.03*
|
Data from experiments like those pictured in Figure 3.
* and ** denote differences in ϵ′ (equal to Ymax,BzATP/Ymax,ATP) between WT and mutant receptors at p<0.05 and p<0.01, respectively.