Figure 6.
Contactin does not change steady-state characteristics of Nav1.3 in transfected HEK-Nav1.3 cells. Normalized current-voltage relationships are shown (A). Steady-state, voltage-dependent inactivation (500 msec prepulses) (B), and recovery from inactivation (C) were determined as described in Materials and Methods. The time course for recovery from inactivation of peak current at -120 mV is shown. Squares, HEK-Nav1.3 cells transfected with GFP. Circles, HEK-Nav1.3 cells transfected with Ctn-GFP. No differences in these properties were seen in HEK-Nav1.3 cells transfected with either Ctn-GFP or GFP. C, Inset, Averaged normalized currents were elicited by a depolarizing pulse from -120 to -10 mV and are overlaid. The currents are indistinguishable from one another.