Figure 7.

Increase in oxidatively modified α-Syn with aging. A,α-Syn was immunoprecipitated from nonionic detergent soluble extracts from young (50s) and old (80s) human SN and young (4 months old) and old (14 months old) WT Huα-Syn Tg mouse brainstem. The levels of protein carbonyls associated with the immunoprecipitated α-Syn were determined by the Oxyblot analysis. Aging is associated with the increased levels of α-Syn-associated protein carbonyls. Also shown are input levels of α-Syn and a control lane showing the results obtained with normal mouse IgG. B, Total protein carbonyl associated with the SDS-soluble proteins from the samples used in A was determined using the Oxyblot analysis. There is a modest age-associated increase in the level of protein carbonyls in human SN. Very little age-associated changes occur with mice. The molecular mass standards are in kilodaltons.