Table 2.
Half-lives of Huα-Syn variants (WT, A53T, and A30P) and Moα-Syn (MoSn) in polyclonal SH-SY5Y cell lines
|
|
Undifferentiated |
Differentiated |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
|
t1/2 fast (%) |
t1/2 slow (%) |
t1/2 total |
R2
|
t1/2 fast |
t1/2 slow |
R2
|
|||||
| WT | 3.8 ± 0.6 (30.8) | 27.7 ± 3.5 (69.2) | 13.0 ± 2.2 | 0.996 | NA | 52.3 ± 3.3 | 0.997 | |||||
| A53T | 3.4 ± 0.8 (31.6) | 29.7 ± 4.0 (68.4) | 13.5 ± 1.5 | 0.991 | NA | 73.6 ± 7.1a | 0.982 | |||||
| A30P | 3.0 ± 0.3 (34.3) | 28.9 ± 2.9 (65.7) | 13.2 ± 1.2 | 1.000 | NA | 50.0 ± 7.8 | 0.964 | |||||
| MoSn |
1.2 ± 0.2 (32.8) |
20.9 ± 3.1 (67.2) |
9.5 ± 1.5b
|
0.999 |
NA |
35.8 ± 2.4c
|
0.950 |
|||||
The values (t1/2, expressed as mean ± SEM in hours; average span, in percentage) are calculated using the same methods as described in Table 1. The best fits (R2) were achieved by assuming that the decays of the α-Syn species are biphasic in the undifferentiated SH-SY5Y cell lines and are monophasic in the neuronally differentiated cells. The differentiation-dependent increases in the half-lives of α-Syn variants were highly significant (p <0.01; t test). Additionally, the half-life of Moα-Syn is shorter than the half-life of WT Huα-Syn in both undifferentiatedb (p <0.05; t test) and differentiated c(p <0.01; t test) SH-SY5Y cells. Finally, the half-life of A53T Huα-Syn is longer than that of WT Huα-Syn in differentiated SH-SY5Y cells a(p <0.01; t test).