Figure 1.

Depletion of mitochondrial (•/○) and cytoplasmic (▪/□) glutathione by 50 μm (•/▪) and 100 μm (○/□) ethacrynic acid in HBSS. Note the earlier and more pronounced depletion of the mitochondrial glutathione pool. Values are shown as mean ± SD. ^*p < 0.05 and ^***p < 0.001, compared with untreated cells (one-way ANOVA with Student-Newman-Keuls test; n = 15-20). Control (100%) values were 1.3 ± 0.3 nmol of glutathione per milligram of mitochondrial protein in the mitochondria and 26 ± 6 nmol of glutathione per milligram of cell protein in the cytoplasm.