Figure 1.
Most GABAergic neurons are also glutamatergic in the AVPV of female rats. A, VGLUT2 mRNA (marker of hypothalamic glutamate neurons detected with 35S-labeled cRNA probes; black grains) and GAD mRNA (marker of GABA neurons detected with digoxigenin-labeled cRNA probes; brown stain) are extensively colocalized in the AVPV nucleus (arrows indicate examples of colocalization). VGLUT1 (marker of extrahypothalamic glutamatergic neurons; black grains) and GAD (brown stain) mRNAs are not found in the same cells in either the CA3 region of the hippocampus (B) or in the sommatosensory cortex (C). Scale bars, 5 μm. 3V, Third ventricle.