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. 2004 Sep 15;24(37):8097–8105. doi: 10.1523/JNEUROSCI.2267-04.2004

Figure 2.

Figure 2.

Sex differences in VGLUT2 and GAD mRNA colocalization. In females (A), but not males (B), most VGLUT2 mRNA (marker of glutamate neurons; black grains) is in GABAergic neurons (brown stain) in the AVPV. However, in the MPO of both females (C) and males (D), most neurons contain either VGLUT2 or GAD mRNA, not both. The black arrows indicate neurons positive for VGLUT2 and GAD mRNAs. The black arrowheads denote cells positive only for GAD mRNA, and the red arrowheads indicate cells positive only for VGLUT2 mRNA. Scale bars, 5 μm. 3V, Third ventricle.