Figure 7.

Effects of E₂ on temporal changes in the number of dual-phenotype IRVGAT/IRVGLUT2 or IRVGAT contacts on medial subpopulations of GnRH neurons. Right, Dual-phenotype terminals were observed in OVX rats that received E₂ (n = 5/group), but not in those that received vehicle (n = 5/group). Dual-phenotype terminals were seen only in animals examined in the afternoon. Left, The number of terminals containing only IRVGAT declined during the period in which LH surge begins in our animal model. ND, Nondetectable. ^***Significantly greater than observed at 9:00 A.M. or in OVX animals (p < 0.001).