Figure 6.

DN-IKKγ protects striatal MSNs against mutant HDx1. A, Living MSNs can be monitored for morphology and Htt expression. Coronal brain slices were cotransfected by biolistic procedures with HDx1-23Q or HDx1-148 and IKKγ or DN-IKKγ. The morphology of transfected cells was monitored by YFP fluorescence (yellow), and Htt expression was monitored by CFP fluorescence (cyan). B, Expression of DN-IKKγ is neuroprotective for MSNs in brain slices. MSN survival was scored 3 d after transfection. Each experiment involved 3-12 transfected brain slices per condition, and the data are means from six independent experiments for each condition.