Figure 1.

PCR products amplified from a leech CNS-specific cDNA library using degenerate primers designed to amplify putative connexins (lanes 1, 2) or putative innexins (lanes 3, 4). In lanes 1 and 3 the PCR reaction mix contained 10% library, whereas in lanes 2 and 4 this was increased to 20% without affecting the results. A bright band of ∼350 bp may be observed in lanes 3 and 4, whereas only primer dimers are seen in lanes 1 and 2. On cloning and sequencing, the 350 bp band was found to be an innexin.