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Photomicrograph of retrogradely labeled cells from the ARH after injection of FG into the MPN. The same field imaged to excite the FITC labeling Y1R (A), tetramethylrhodamine (TRITC) labeling β-END (B), and FG-labeling efferent projections (C) from the MPN. The images are combined in D. Doubly labeled β-END and Y1R immunoreactivity are yellow (arrowheads), and triple-labeled are white (asterisk).
