Table 3.

Real-time PCR validation of the microarray changes

Region	Microarray	Real-time PCR	Accession number	Gene name (BLAST)
	▴	▴	AA161816	Apoptosis inhibitory protein 5
	▴	▴	AI893666	Shroom
	▴	▾	W08432	Brain protein 44-like protein
	▴	▾	AA543916	Unc-51 serine/threonin kinase
	▾	▾	AA241132	Coatomer protein complex, subunit g1
	▾	▴	W13905	Fibrinogen/angiopoietin-related protein
	▾	▴	AA200282	Tristetraproline
	▾	▾	AA207759	Cleavage stimulation factor
	▾	▾	AA097901	Asparaginase like
	▾	▾	AA272747	Nuclear factor, erythroid-derived 2
CA3	▾	▾	W59380	EST
	▴	▴	W99102	Prion protein
	▴	▴	AA184845	Sos homolog 2
	▴	▾	AA146090	EST
	▾	▾	W11965	Enolase 3, b
	▾	▴	AA050726	Paired mesoderm homeobox 1
	▾	▾	AA066202	Adducin 3g
	▾	▾	AA176045	Forkhead box C2
	▾	▾	AA000819	Sphingosine kinase 1
	▾	▾	AA208297	FXYD domain-containing ion transport regulator 2
Dentate	▾	▾	AA215144	Neighbor of TID protein 2

Clones were selected randomly from those maximally differentially expressed between active caspase 3(+)/TUNEL(+) and a nonlabeled neuron in an uninjured brain from both the CA3 subfield and dentate. The columns represent the direction of differential expression in either microarrays or real-time PCR, resulting in an agreement of 71%. The GenBank accession numbers are listed for the ESTs spotted on the array, in addition to the resulting gene names from the corresponding Basic Local Alignment Search Tool (BLAST) search (as of December 2002).