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. 2004 Mar 24;24(12):3136–3146. doi: 10.1523/JNEUROSCI.4506-03.2004

Figure 2.

Figure 2.

LacZ staining and GDNF expression in adult double-transgenic mice. A, LacZ staining in a coronal section through the striatum in a double transgenic mouse. The striatum (STR) is intensely positive for a reaction product. Staining is also observed in cortex, primarily in superficial and deep layers. B, Western analysis of GDNF expression in double-transgenic mice resulting from a cross of line 14 BiTetO-LacZ-rGDNF monotransgenic mice with CaMKIIα-tTA monotransgenic mice. In the top panel, more dimeric GDNF is present in forebrain in a double-transgenic mouse than in a littermate wild-type control or in a double transgenic resulting from a line 2 founder cross, which lacked LacZ staining. In the bottom panel, a line 14 double-transgenic mouse expresses more GDNF than a line 12 double-transgenic mouse that lacked LacZ staining or a line 12 BiTeto-LacZ-rGNDF monotransgenic control. The double transgenics express approximately twofold to threefold more GDNF in forebrain than controls. ND, Not determined.