Figure 9.

Cortical dopaminergic innervation in CBLG-DT mice. A, B, Representative examples of DAT immunostaining in piriform cortex in a double-transgenic animal (B) in comparison with a wild-type animal (A). In the double-transgenic animal, there are many more extended and branched DAT-positive fibers (arrows). By stereological analysis, there are also more DAT-positive boutons in the cortex of double-transgenic (DT) animals (C; n = 6, each group; p = 0.007). D, E, Representative examples of TH immunostaining of fibers entering the cingulate cortex (CCTX) from the corpus callosum (CC) in a double-transgenic animal (E) in comparison with a wild-type animal (D). Quantitative analysis confirmed that TH-positive fibers were more numerous in DT mice compared with WT and BiTetO-LacZ-rGDNF monotransgenic (MT) mice (n = 3, each group; p = 0.004, ANOVA). Scale bar, 100 μm