Figure 3.

Lack of increase in IκB-α phosphorylation in cortical neurons after DNA damage. A, Positive control for Ser³² phosphorylation; fibroblasts were treated with TNF (10 μm) for the indicated times and analyzed by Western blot analyses, using a phospho-epitope-specific IκB-α antibody. Total cell lysates of cultured cortical neurons also were treated with camptothecin alone (B) or camptothecin and the proteasomal inhibitor ALLN (C; 50 μm) for the indicated times and analyzed for Ser³² IκB-α phosphorylation as above.