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. 2004 Jul 21;24(29):6553–6562. doi: 10.1523/JNEUROSCI.0886-04.2004

Figure 9.

Figure 9.

Effects of KCl and TTX on MCN neurite density in vitro. A-H, Representative immunohistochemical views of immunoreactive OT (A, C, E, G) and VP (B, D, F, H) MCN fibers found adjacent to the SON in rat hypothalamic slices cultured for 15 d. Control SON slices cultured in the absence of any treatment (A, B), SONs cultured in the presence of 25 mm KCl (C, D), SONs cultured in the presence of 2.5 μm TTX (E, F), and SONs cultured in the presence of both 2.5 μm TTX and 25 mm KCl (G, H) are shown. Scale bar, 100 μm. Note that the deleterious effect of TTX on MCN neurite outgrowth (C, D) was reversed by the simultaneous application of KCl in the media (G, H).