Figure 10.

Antidepressant imipramine (Im) induces an increase in synaptic GluR1 in vivo. Rats were treated with imipramine (10 mg/kg, twice daily, i.p.) for 4 weeks; hippocampal tissues were isolated, and synaptosome preparations were obtained by using a Ficoll gradient assay. A, B, Equal amounts of proteins were separated by gel electrophoresis and analyzed with anti-GluR1 antibodies and anti-synaptophysin antibodies. The bands were analyzed by the Kodak Imaging System (n = 8; t test; ^*p < 0.05). Con, Control.