Figure 5.

Duration of fosGFP fluorescence after in vivo stimulation. A-C, fosGFP transgenic animals were injected with hypertonic saline as a dehydration stimulus and simultaneously deprived of water for 2 hr, and fosGFP expression (A) was examined in the PVN at this time. Water was reintroduced ad libitum, and fosGFP signal is shown at 2 (B) and 6 (C) hr after water reintroduction. D, The number of fosGFP⁺ cells at each time point was calculated to determine the duration of fosGFP⁺ cells in the PVN after stimulus resolution. Error bars represent SE. 3v, Third ventricle. Scale bar, 100 μm.