Figure 3.

EMSA analysis of CREB binding in nuclear extracts from hippocampal neurons and astrocytes. A, Hippocampal neurons or astrocytes were untreated or treated with IL-1 (10 ng/ml) for 1 hr. EMSA analysis demonstrated binding to CRE in neurons, but not in astrocytes. B, Supershift assay. Nuclear extracts from IL-1-treated hippocampal neurons were incubated with the labeled CRE oligonucleotide and antibodies against CREB or ATF-2. Nuclear extracts from PC12 cells were used as control; competition with excess unlabeled nonspecific (NS) or specific (S) oligonucleotide probe demonstrates the specificity of the binding.