Figure 5.

Fura-2 analysis of intracellular calcium. A, Representative traces of kainate-induced (100 μm) calcium responses recorded from cultured hippocampal neurons; kainate (KA) or NMDA (30 μm) was applied for ∼10 sec (bars above traces), inducing pronounced increase in internal calcium as determined by increase from baseline ratio (25> baseline ratio; n = 15 for KA and n = 20 for NMDA). B, IL-1 applied for >1 min on cells that previously responded to KA or NMDA failed to induce significant changes from baseline ratios (n = 35). C, ATP (30 μm) applied for 10 sec to cells that failed to respond to IL-1 displayed pronounced increase in internal calcium (20> baseline ratios; n = 35).