Figure 4.

BzATP does not permeabilize RGCs and Muller cells. A, RGCs were labeled with micro-ruby (red). Crystals of the dye were applied on the nerve stump of dissected eyeballs for 15 min. After washout, the retina was dissected and kept for 2 hr at 32°C to allow retrograde transport of the dye to the RGC bodies. When YO-PRO-1 and BzATP were applied, no micro-ruby-labeled cells allowed the entry of YO-PRO-1 (blue). B, Muller cells were labeled by perfusion of red Mitotracker (10 mg/ml) for 2 min in the extracellular saline. Bright-stained Muller cell end feet (red) in the ganglion cell layer do not overlay with the YO-PRO-1 labeling (blue) induced by BzATP stimulation. Scale bars, 50 μm.