Figure 3.

Diagram and effects on egg laying of wild-type and mutant egl-47 transgenes. A, Diagram of egl-47 transgenes. Stop codons are shown that were included in certain transgenes so that one, both, or neither of the isoforms could be expressed. Also shown is the n1081dm gain-of-function (gf) mutation, included in some transgenes. The egl-47 coding exons are indicated by boxes; A-specific exons are black; the B-specific exon is white; common exons are gray. In addition to the coding regions shown, the transgenes contained 4.8 kb of 5′ promoter and 0.7 kb of 3′ untranslated region DNA. B, Effects of the transgenes. Indicated are the mutations included in individual transgenes, concentrations of DNA injected, and the number of Egl lines produced. Lines were defined as Egl if >20% of adults contained at least 30 eggs. When EGL-47 activity was increased, by overexpression or by mutation, egg laying was inhibited. Either isoform was capable of inhibiting egg laying.