Figure 3.

The LN_ds of pdf⁰¹ mutants phase advance in DD. Please refer to Figure 2 for details on figure format. In the seneurons, cytoplasmic PER is seen at CT17 of control flies but earlier in pdf⁰¹ mutants. Across all DD days, the LN_ds have predominantly nuclear PER at CT5 and CT23. Low PER staining in control brains stained at CT13 of DD3 precluded identification of LN_ds. An average of 13 hemispheres are represented in each time point. All plots showed statistically significant rhythms by ANOVA testing (p < 0.05). An asterisk on the representative image of y w on CT5 of DD9 marks a sinus-like feature, which has not been described previously, around which the LN_ds were consistently observed.