Figure 4.
PER intensity rhythms persist in the small LNvs of pdf01 mutants. Confocal images from a subset of brains are shown; quantified intensity values are shown in the graphs to the right. At some time points, PER staining was too low to allow for reliable identification of small LNvs. In these panels, such as CT11 of DD day 3 in control flies, blank squares exist to indicate that fewer than eight examples could be found. On average, 15 brain hemispheres per time point were imaged. All plots showed statistically significant rhythms by ANOVA testing (p < 0.05). In the graphs, the y-axis represents absolute intensity values of a maximum of 4095. x-axis represents CT. Markings denote statistically equivalent time points by the Tukey multiple comparison test: boxed time points have peak values, and bracketed time points have trough values.