Figure 1.

Morpholinos are retrogradely transported into neuronal somata in the brainstem and reduce L1.1 immunoreactivity. a-d, In a cross section through the NMLF, the number of L1.1-immunopositive neurons is increased 10 d after a lesion (b) compared with unlesioned controls (a). Upregulation is greatly reduced when L1.1 morpholino is applied to the spinal lesion site (c). In the same tissue section (d), accumulation of fluorescein-tagged L1.1 morpholino is visible with the appropriate filters. e, The number of L1.1-immunoreactive cell profiles is reduced by L1.1 morpholino compared with control morpholino-treated animals in a concentration-dependent manner (^*p < 0.05). Scale bar: (in d) a-d, 50 μm. Error bars represent SEM.