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. 2004 Sep 8;24(36):7931–7938. doi: 10.1523/JNEUROSCI.2115-04.2004

Figure 5.

Figure 5.

Cl- uptake mechanisms in mouse OSNs. A, 2P-FLIM images of the epithelial surface during transient exposure to 50 mm extracellular Cl-. [Cl-]i in dendritic knobs declined when extracellular Cl- was reduced from 150 to 50 mm and recovered after high Cl- was restored (a-c). Bumetanide (BU) (50 μm) prevented recovery (d-f) as did exposure to Na+-free solution (g-j). Scale bar, 10 μm. B, Quantitative analysis of [Cl-]i of the experiment shown in A. The light gray area indicates the time interval when extracellular Cl- was reduced from 150 to 50 mm. Reuptake of Cl- into the dendritic knobs was suppressed by bumetanide and by exposure to Na+-free solution. Na+-dependent, bumetanide-sensitive Cl- uptake represents evidence for the activity of a NKCC-type Cl- transporter. Mean ± SEM of five to eight knobs. C, Detection of Cl- transporter mRNA in rat olfactory epithelium. RT-PCR experiments yielded signals for KCC1, NKCC1, and NCC in olfactory epithelium cDNA (OE). No expression of KCC2 or NKCC2 was detected. Positive controls for KCC2 [rat hippocampus (HC)] and for NKCC2 [rat kidney (K)] are shown on the right.