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. 2004 Oct 6;24(40):8752–8761. doi: 10.1523/JNEUROSCI.3155-04.2004

Figure 4.

Figure 4.

Colocalization of mGluR5a with clathrin and mGluR1a in HEK 293 cells. a, Representative laser scanning confocal micrograph showing the colocalization (yellow) of FLAG-mGluR5a labeled with Alexa Fluor 555-conjugated monoclonal FLAG antibody (red) with GFP-clathrin (green). Cell surface-labeled receptors were allowed to endocytose for 20 min at 37°C. b, Representative laser scanning confocal micrograph showing the colocalization (yellow) of FLAG-mGluR1a labeled with Alexa Fluor 555-conjugated monoclonal FLAG antibody (red) with mGluR5a-GFP (green). Cell surface-labeled receptors were allowed to endocytose for 20 min at 37°C. Scale bars, 10 μm.