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The mitochondrial response to βA but not the change in [Ca2+]c is mediated by the NADPH oxidase. Astrocytes were co-loaded with fura-2 and Rh123 for simultaneous measurement of [Ca2+]c and Δψm. Cells were preincubated with 0.5 μm DPI for 30 min. [Ca2+]c transients were seen as usual after application of 50 mm βA, whereas the mitochondrial response was suppressed completely.
