ephrin expression is normal after Sema3D knock-down. A-D, In situ hybridization for ephrin-A5. A, B, Lateral views of eyes of 48 hpf conMO-injected (A) and 3DMO-injected (B) embryos. ephrin-A5 is expressed normallly in the nasal retina (and in lens) in both conMO- and 3DMO-injected embryos. N, Nasal; T, temporal; D, dorsal; V, ventral. C, D, Dorsal views of the midbrain of 48 hpf conMO-injected (C) and 3DMO-injected (D) embryos showing ephrin-A5 normal expression in the posterior tectum. A, Anterior; P, posterior. E-H, In situ hybridization for ephrin-B2. E, F, Lateral views of eyes of 48 hpf conMO-injected (E) and 3DMO-injected (F) embryos showing that ephrin-B2 is expressed normally in the dorsal retina (and the lens). G, H, Dorsal views of right tectum showing ephrin-B2 expression along dorsal midline of tectum in conMO- and 3DMO-injected embryos. Arrows indicate dorsal midline. Scale bars, 50 μm.