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. 2019 Aug 30;13:917. doi: 10.3389/fnins.2019.00917

FIGURE 1.

FIGURE 1

FAM19A5-LacZ knock-in (KI) gene construct and FAM19A5 expression in the nervous system at developmental stages. (A) Schematic diagram of the FAM19A5-LacZ KI mouse gene construct. The target vector containing the LacZ gene is inserted in front of exon 4 (E4) of the FAM19A5 gene by homologous recombination, producing β-galactosidase enzyme under control of the FAM19A5 gene promoter. (B) Whole embryo X-gal staining at embryonic days E10.5, E12.5 and E14.5 of FAM19A5-LacZ KI homozygote (+/+) mice. X-gal staining occurred from the early embryonic stage E10.5, with increased and continuous staining in the later embryonic stages. (C) Comparison of sagittal views between X-gal staining (left) and in situ hybridization pattern (right) at E14.5. Mouse and rat embryos at E14.5 were subjected to X-gal staining and in situ hybridization, respectively, revealing a similar pattern of expression. (D) FAM19A5 expression in the germinal zones; ventricular zone (VZ), subventricular zone (SVZ) and ganglionic eminence (GE). Coronal view of E12.5, E14.5, E16.5, and E18.5 X-gal–stained sections. (E) X-gal staining for coronal brain sections at postnatal stages P4.5, P10.5, P15.5, and P20.5, suggesting broad and continuous FAM19A5 expression throughout the postnatal stages. aca, anterior commissure, anterior part; AON, anterior olfactory nucleus; BLA, basolateral amygdaloid nucleus, anterior part; CB, cerebellum; cc, corpus callosum; CP, caudate putamen; drg, dorsal root ganglion; ENT, entorhinal cortex; EPd, dorsal endopiriform nucleus; E1, exon 1; E2, exon 2; E3, exon 3; E4, exon 4; fa, forceps minor of the corpus callosum; fb, fore brain; GE, ganglionic eminence; gl, glomerular layer of the olfactory bulb; gr, granular layer of the olfactory bulb; hb, hind brain; HIP, hippocampal region; int, internal capsule; IRES, internal ribosome entry site; LV, lateral ventricle; mb, mid brain; MZ, marginal zone; neo, neomycin phosphotransferase gene; OT, olfactory tubercle; PIR, piriform cortex; SA, splice acceptor site; sc, spinal cord; SVZ, subventricular zone; TH, thalamus; VZ, ventricular zone; WT, wild-type. Ventricles (lateral ventricle, 3rd ventricle and 4th ventricle) are demarcated by dashed lines in panel C-E. All animals used were FAM19A5-LacZ KI mouse model otherwise labeled. Scale bars represent 2 mm for panel B, 500 μm for panels C and E, and 100 μm for panel D.