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. 2019 Aug 30;13:917. doi: 10.3389/fnins.2019.00917

FIGURE 2.

FIGURE 2

X-gal staining of the adult FAM19A5-LacZ knock-in (+/+) mouse brain. (A) X-gal staining of coronal sections of 10-week old adult male mice. The section’s distance from the Bregma point is indicated at the upper right hand corner of each box. The lateral ventricle, third ventricle, fourth ventricle, and aqueduct of Sylvia are demarcated by dashed lines. (B) X-gal staining combined with 3, 3′-diaminobenzidine staining on adult mouse brain sections. Brain sections incubated in X-gal staining solution for 24 h were immune-labeled for various cellular markers: NeuN for neuron, GFAP for astrocyte, NG2 for oligodendrocyte precursor cell, and Iba1 for microglia. X-gal signals (blue) are seen in subpopulations of NeuN+, GFAP+, NG2+, and Iba1+ cells in the adult mouse brain. Arrows and arrow heads depict cell marker positive cells with and without X-gal signals, respectively. The areas for the corpus callosum are indicated by dashed lines. aca, anterior commissure, anterior part; AON, anterior olfactory nucleus; BLA, basolateral amygdaloid nucleus, anterior part; CA1, field CA1 of the hippocampus; CA2, field CA2 of the hippocampus; CA3, field CA3 of the hippocampus; CB, cerebellum; cc, corpus callosum; CP, caudate putamen; CTX, cerebral cortex; DG-po, polymorphic layer of the dentate gyrus; ENT, entorhinal cortex; gl, glomerular layer of olfactory bulb; gr, granular layer of olfactory bulb; H, habenula; HIP, hippocampal region; HY, hypothalamus; int, internal capsule; IC, inferior colliculus; MGv, medial geniculate nucleus, ventral part; OT, olfactory tubercle; PIR, piriform cortex; SC, superior colliculus; SN, substantia nigra; so, stratum oriens of the hippocampus; TH, thalamus. Scale bars represent 500 μm in panel A and 50 μm in panel B.