FIGURE 6.

Nuclear X-gal precipitation in Sez6l2⁺ neurons and NG2⁺ oligodendrocyte precursor cells of the TBI-induced brain. (A) Fluorescence and differential interference contrast (DIC) images of the cortical region in naïve and TBI7D brain sections after X-gal staining combined with Sez6l2 immunostaining and Hoechst staining. Boxed regions are magnified in the right panels showing nuclear morphology and X-gal staining pattern. (B) Fluorescence and DIC images of NG2⁺ OPCs in the corpus callosum. Nuclear X-gal was stained in NG2⁺ OPCs of the TBI-induced group. Boxed regions are magnified in the right panels showing nuclear morphology and X-gal staining pattern. (C) Fluorescence images for X-gal staining combined with GFAP, nestin, Iba1, NG2, CD31, or PDGFRβ immunostaining of the penumbra and corpus callosum of TBI7D brain section. Arrows and arrowheads indicate cell type–specific marker⁺/nuclear X-gal^– and cell type–specific marker^–/nuclear X-gal⁺ cells, respectively. Scale bar, 10 μm.