Figure 10.
Immunostaining for Kv1.2 subunit in soma and dendrite of M-cell. Polyclonal anti-rat Kv1.2 antibody (Alomone Labs) was used. A–E, Serial horizontal sections with 50 μm thickness where the lateral dendrite (A–C, arrows), soma (D, arrow), and proximal ventral dendrite (E, arrow) of the M-cell were immunolabeled. Fibers that appeared to connect to the distal lateral dendrite were also immunolabeled (A, B, asterisks). F, Viewing fields in A–E. G–I, Horizontal sections of hindbrain, including the labeled M-cell (arrowheads). The soma of the M-cell was observed in G, and the ventral dendrite of the M-cell was observed in H and I. H and I were 100 and 150 μm ventral to G, respectively. Dorsal RS neurons caudal to the M-cell, including MiD2cm and MiD3cm, were not labeled. Some mlf fibers were labeled (brackets). Arrows indicate the midline.