Figure 4.

Agarose gel electrophoresis of reverse transcriptase PCR products for Cx36 mRNA. A, Micrograph of the ONL of wild-type mouse retina. The dotted line separates the inner, rod-containing half of the ONL (IH) and the outer half (OH), which is composed of both rods and cones. B, Cx36 mRNA was detected in samples taken from the outer half of the ONL. The molecular weight of the amplicon corresponds to the expected value (447 bp). C, Samples harvested from the inner half of the ONL were negative for Cx36 mRNA. Ret, Positive control performed with transcripts from whole mouse retina. Scale bar: A, 10 μm.