Figure 5.

Formation of a stable Ncad-GFP punctum. a, In addition to the mobile fluorescent puncta present in the developing Rohon-Beard axons, there are also stable puncta. The stable accumulations (arrowhead), as shown in this time-lapse sequence, form soon after the departure of the migrating axonal growth cone. Scale bar, 10 μm. b, Cumulative probability of stable punctum formation. The formation of multiple stable puncta were characterized relative to the axonal growth cone. The fraction of puncta formed was plotted as a function of time after initial growth cone arrival. The plot shows that nearly all stable Ncad-GFP accumulations form within 1 hr after arrival of the growth cone, with an average time to formation of 32 min.