. 2004 Oct 20;24(42):9405–9413. doi: 10.1523/JNEUROSCI.0899-04.2004

Table 2.

Fos expression in the spinal cord 5 d after acid injection

	Wild type			High-OE myo/NT-3
	Contralateral	Ipsilateral	Contralateral	Ipsilateral	Contralateral	Ipsilateral
Lamina I-II
Saline	1.40 ± 0.23	3.60 ± 1.27	3.60 ± 1.60	4.13 ± 2.53
Acid	1.73 ± 0.64	14.87 ± 1.05^*	3.07 ± 1.88	3.07 ± 1.20^**	1.0 ± 0.32	1.2 ± 0.49
Lamina III-IV
Saline	3.07 ± 0.84	3.60 ± 0.53	9.40 ± 5.20	9.73 ± 5.25
Acid	5.27 ± 0.74	12.93 ± 1.73^*	7.87 ± 3.99	8.27 ± 2.97	1.2 ± 0.37	1.4 ± 0.51
Lamina V-VI
Saline	8.07 ± 1.13	7.93 ± 0.88	21.33 ± 12.08	22.60 ± 10.60
Acid	12.53 ± 1.27	23.00 ± 1.21^*	15.93 ± 7.53	17.87 ± 5.97	1.0 ± 0.55	0.6 ± 0.24

The number of Fos-positive cells was counted from different spinal laminas from both ipsilateral and contralateral sides of the spinal cord 5 d after acid injection. Comparisons were made between groups using one-way ANOVA and Fisher's PLSD. In regard to NT-3 overexpression, wild-type mice were derived from litters within the myo/NT-3 colony. For exogenous treatments, wild-type CF-1 mice were purchased from Charles River. In wild-type mice, acid injection caused significant increases in the number of Fos-positive neurons compared with saline-injected mice in each lamina (^*p < 0.05). In contrast, acid treatment did not increase Fos-positive neurons in high-OE myo/NT-3 as well as in wild-type mice treated with NT-3. Moreover, the number of Fos-positive nuclei was significantly lower in acid-injected high-OE myo/NT-3 mice compared with acid-injected wild-type mice (^**p < 0.05). Data are plotted as means ± SEM; n = 3 animals per group.