Figure 6.

Synaptic protein synthesis of tPA induced by glutamate. A, The SN fraction was isolated from the hippocampus and stimulated with increasing concentrations of glutamate for 30 min, then tPA was analyzed by zymography. B, SNs were pretreated with AD, CD, and CX before and during glutamate (100 μm) stimulation as in A. C, tPA synthesis is dependent on the activation of mGluR. All glutamate receptor antagonists were added 5 min before glutamate stimulation as in B. D, The type I mGluR agonist DHPG mimics glutamate stimulation; however, the group II mGluR agonist APDC does not increase tPA levels. ^*Statistically significant difference from control (CON); #statistically significant difference from glutamate-stimulated group (p < 0.05; mean ± SEM; n = 4 representative zymograms shown above each graph).