Figure 3.

The Shank1 SH3 domain binds to the ankyrin repeat domain (ANK). A, Diagram of the wild-type and mutant constructs of Shank1B (all HA-tagged at the N terminus) used in this study. B, Extracts of HEK cells transfected with wild-type Shank1B or mutant Shank constructs (as indicated above the Western blot) were incubated with GST or GST-SH3, and the pull-down proteins were revealed using HA antibody. In the input lane, ∼10% of the total extract used for the pull-down was loaded. C, GST-SH3, but not GST alone, can pull down purified His₆-ANK domains.