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. 2004 Nov 24;24(47):10642–10651. doi: 10.1523/JNEUROSCI.3584-04.2004

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Copyright © 2004 Society for Neuroscience 0270-6474/04/2410642-10.00/0

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Figure 6. — Regional clearance of cholesterol deposits at 2 weeks after transplantation visualized by filipin staining in the ASMKO mouse brain that received NPCs. A, Coronal view of the adult mouse hippocampus showing the broad dispersion of transplanted BrdU-immunoreactive cells. B, Adjacent sections stained with filipin showing that the regional clearance of fluorescent deposits matches closely with the distribution of the transplanted cells indicated by arrowheads. D, Higher magnification showing the substantial decrease of filip in deposits within the granular cell layer (gcl), the molecular layer, and the CA1 region of the hippocampus from the transplanted region (indicated by arrowheads in B) compared with the nontransplanted side (C). Scale bars: (in A) A, B, 200 μm; (in C) C, D, 100 μm.