Figure 12.

BDM and ML-7 decrease bidirectional transport of GFP-tagged particles. A, Sequential images from two representative transfected cells captured at the indicated intervals before and after the addition (arrow) of 20 mm BDM. Small arrows denote representative GFP-tagged structures. The accompanying graphs present the percentage of particles displaying either red or green fluorescence versus those displaying orange-yellow fluorescence. Note that ML-7 reduced the percentage of translocating particles. B, The graph depicts the percentage (mean ± SEM; n ≥3 neurites under each condition from multiple experiments) of GFP-tagged structures undergoing anterograde transport, retrograde transport, or exhibiting no motion either before or 25-30 min after the addition of ML-7 or BDM as described in A. Note that both ML-7 and BDM reduce the bidirectional transport of GFP-tagged structures.