Figure 7.

Mutation of Ser1480 to glutamate decreases surface expression of NR2B in neurons. A, Surface immunostaining of GFP-NR2B wild-type and mutant subunit (NR2B S1480E) in which Ser1480 was replaced with glutamate to mimic phosphorylated Ser1480 (left; scale bar, 10 μm). The right panels show higher magnification of the insets in the left panel. B, In the yeast two-hybrid system, PDZ domains of PSD-95 failed to interact with phosphorylation mimic mutant NR2B S1480E and deletion mutant NR2B (NR2B Δ5aa), in which the PDZ ligand was deleted. C, Quantification of surface GFP staining of wild-type NR2B (n = 19) and mutant NR2B S1480E (n = 26) as the ratio of surface GFP intensity over total GFP intensity per unit area (t test; ^***p < 0.001).