Figure 10.

Pressure-induced ischemia of guinea-pig retinas causes morphological alterations that are similar to those observed during acute exposure to glutamate. Immediately after the end of a 1 hr elevation of the intraocular pressure above the systolic blood pressure, the retinas were acutely isolated and the Müller glial cells were labeled with Mitotracker Orange. A, Left, The views of the deep IPL display thinner Müller cell profiles (light round structures) in the ischemic retina compared with the control retina. Right, Mean cross-sectional area of the glial cell profiles in the IPL. B, Mean cross-sectional areas of neuronal cell bodies within the GCL-NFL. C, Acutely isolated ischemic retinas are thicker as control retinas. Left, Examples of retinal slices; the intrinsic optical signals were recorded. Right, Mean thickness of the IPL in retinal slices (in micrometers). The data were obtained in three independent experiments. ^•p < 0.05. Scale bars, 25 μm.