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. 2004 Nov 10;24(45):10074–10083. doi: 10.1523/JNEUROSCI.2604-04.2004

Figure 6.

Figure 6.

Host reponse in the presence of IFN-γ. A, C, E, G, I, Tumors from double transgenic animals released from doxycycline on E16. B, D, F, H, J, Tumors from double transgenic animals released from doxycycline on E16 and then retreated with doxycyline on P16 to repress expression of the IFN-γ transgene. A, B, Hematoxylin and eosin staining shows extensive necrosis in the tumors of mice with continued expression of IFN-γ. C, D, PCNA immunostaining shows many more proliferating cells (arrows) in the tumors of mice with continued expression of IFN-γ. E, F, TUNEL staining shows numerous dying cells (arrows; green fluorescence) in the tumors of mice with continued expression of IFN-γ, with weak blue fluorescence showing DAPI counterstain. G, H, MAC-1 immunostaining reveals larger numbers of microglia/macrophages (arrows) infiltrating the tumors of mice with continued expression of IFN-γ. I, J, CD3 immunostaining reveals numerous CD3+ T-cells (arrows) infiltrating the tumor of mice with continued expression of IFN-γ. Scale bars: A, B, E-H, 75 μm; C, D, 25 μm; I, J, 50 μm.