Figure 4.

Widespread NaGlu activity in treated MPSIIIB mouse brains. MPSIIIB mice received a single injection of AAV2-NaGlu or AAV5-NaGlu vectors in the right putamen at 6 weeks of age and were analyzed at the indicated time. One-millimeter-thick coronal brain slices were prepared from the injected (IL) and noninjected (CL) hemispheres. Brain maps of NaGlu activities for each analyzed mouse are shown. Strips on brain maps schematically represent coronal slices (slice number is shown on the left). Values shown in the strips are NaGlu activities measured in the slice homogenate (in units per milligram of protein). Zero indicates that activity was <0.1 U per milligram of protein. Strips corresponding to slices in which NaGlu activity was detected are shadowed in gray according to the code shown at the bottom. Strips without values correspond to brain slices not used for NaGlu assay. Mouse reference numbers are indicated, as well as a letter code indicating other investigations performed on the same mouse: H, Histopathology; G, ganglioside assay (not all mice in which gangliosides were investigated are shown); B, behavioral testing (1 mouse tested in the open field is not shown here); P, quantitative PCR amplification of vector genomes.