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. 2004 Dec 1;24(48):10918–10923. doi: 10.1523/JNEUROSCI.4020-04.2004

Figure 3.

Figure 3.

RhoA inhibition antagonizes both the suppression of Ca2+ current amplitude and its kinetics changes triggered by the sJMD. A, Peak calcium current traces recorded at 2 min (top trace) and at 5 min (bottom trace) while perfusing the neuron with sJMD (1 μm) and C3 exoenzyme (500 nm). Note that C3 reverts the calcium current inhibition caused by the sJMD. B, Average current density-voltage plots for control (n = 10), sJMD (n = 9), and sJMD plus C3 exoenzyme (n = 7) recordings. Note that inhibition of RhoA recovers the calcium current density. Inset, Superimposed peak currents for control, sJMD, and sJMDp plus C3 exoenzyme. C, Raw data fitted to a Boltzmann function. Note that the rescue of current amplitude by C3 does not involve a change in the voltage dependence of the ion channel steady-state inactivation (control: Vmid = -8.9 ± 0.4 mV, Vc = -3.1 ± 0.4 mV; sJMD: Vmid = -9.1 ± 0.3 mV, Vc = -3.2 ± 0.3 mV; sJMD plus C3: Vmid = -9.0 ± 0.3 mV, Vc = -3.0 ± 0.3 mV). D, Ten to 90% activation time. E, Iend/Ipeak inactivation index. F, Tau tail deactivation parameter, as a function of voltage step. Note that inhibition of RhoA opposes all current changes attributable to the sJMD. Values are expressed as mean ± SE.