Figure 4.
ROCK inhibition counteracts Ca2+ current suppression attributable to sJMD. A, Peak calcium current traces recorded at 1 min (top trace) and at 2 min (bottom trace) while perfusing with sJMD (1 μm) and Y-27632 (10 μm). Note that the ROCK inhibitor reverts current inhibition caused by the sJMD. B, Examples of superimposed calcium currents recorded in controls (•) and in the presence of sJMD (○) or sJMD plus Y-27632 (▵). Note that the inhibition of ROCK in the presence of the sJMD leads to a substantial recovery in ICa amplitude. C, Average peak current density for control (62.3 ± 4.1 pA/pF; n = 10), sJMD (27.9 ± 3.0 pA/pF; n = 9), and sJMD plus Y-27632 (41.4 ± 2.2 pA/pF; n = 7). Note that Y-27632 counteracts sJMD inhibition of average peak calcium current density. Values are expressed as mean ± SE.