Figure 4.

2meSATP did not increase IPSC frequency in CA3 pyramidal neurons from the P2Y₁-deficient mice. A, Membrane current traces recorded with CsCl-based internal solution in the presence of kynureic acid (1 mm) from a CA3 pyramidal neuron from a rat (top traces), a wild-type mouse (WT; middle traces), and a P2Y₁-deficient mouse (P2Y₁-/-; bottom traces). Traces obtained before (left) and after (right) 2meSATP (100 μm) application. The holding potential was -70 mV. B, The time course of the changes in IPSC frequency with 2meSATP application. Mean and SE of nine neurons from eight slices from four wild-type mice (open circles) and 14 neurons from eight slices from four P2Y₁-deficient mice (filled circles). IPSC frequency became significantly different (p < 0.05; ANOVA) between in the wild-type and in the P2Y₁-deficient mice during the period indicated by the horizontal bar below the plots. C, Left, Summary of the effects of 2meSATP on IPSC frequency from wild-type mice in the absence and presence of MRS2179 (30 μm). ^*p < 0.05; ^**p < 0.01; NS, not significantly different (ANOVA); n = 8 from four wild-type mice. Right, Summary of the effects of 2meSATP on IPSC frequency recorded in P2Y₁-deficient mice. NS, Not significantly different (ANOVA); n = 14 from four P2Y₁-deficient mice.