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Phosphorylation of 4EBP, p70S6K, and S6 in dendrites induced by BDNF. Phosphorylation of 4EBP (A-C), p70S6K (G-I), and S6 (P-R) in control (A, G, M), BDNF (100 ng/ml)-treated (B, H, N), and BDNF plus rapamycin-treated (C, I, O) neurons was detected by immunocytochemistry with anti-phospho-specific antibodies (red fluorescence). Double immunostaining was performed with anti-MAP2 antibody (green fluorescence). Scale bars, 20 μm.
