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. 2004 Nov 3;24(44):9760–9769. doi: 10.1523/JNEUROSCI.1427-04.2004

Figure 7.

Figure 7.

Effects of mTOR siRNA on mTOR protein levels and the phosphorylation state of p70S6K. Western blotting of mTOR and p70S6K, together with glyceraldehyde-3-phosphate dehydrogenase (GAPDH), under the presence or absence of mTOR siRNA with or without BDNF is shown. Phosphorylation of mTOR and p70S6K was also examined. Neurons incubated with control or mTOR siRNA for 48 hr were treated with or without BDNF for 5 min (A). BDNF-induced protein synthesis is inhibited by mTOR siRNA (B). Results of densitometric analysis are shown in C. Cont, Control; Si, siRNA. Bars represent mean + SD. *p < 0.005 (t test) (mTOR, n = 8; P-mTOR and P-p70S6K, n = 4). GAPDH and p70S6K levels are not changed by mTOR siRNA.