Figure 7.

Cytoskeletal perturbation and signaling inhibitors differentiate types of phagocytosis. A, Nocodazole (5 μg/ml) was added to BV-2 cells to inhibit microtubule dynamics 45 min before stimulation with fAβ25-35 (60 μm; 63.6 μg/ml), immune IgG (1 mg/ml), and mouse complement-opsonized zymosan (mOZ) (1 mg/ml). B, Blebbistatin (100 μm) was added to BV-2 cells 30 min before stimulation with the indicated ligand. C, Piceatannol (10 μm), a Syk inhibitor, PP2 (10 μm), a Src inhibitor, and LY294002 (10 μm), a PI3K inhibitor, were added to BV-2 cells 30 min before stimulation with the indicated ligand. The data are representative of five independent experiments. ^**p < 0.001 compared with control.